Because Hoechst stains bind to DNA, they interfere with DNA replication during cell division. Consequently, they are potentially mutagenic and carcinogenic, so care should be used in their handling and disposal. Hoechst stain is used to sort sperm in livestock and humans. Its safety has been debated. Se mer Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33,342nd … Se mer • Bisbenzimide • Carcinogen • DAPI • DNA-binding protein Se mer Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue-cyan fluorescent light around an emission spectrum maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510–540 nm range. Hoechst stains can be … Se mer A concentration of 0.1–12 μg/ml is commonly used to stain DNA in bacteria or eukaryote cells. Cells are stained for 1-30 min at room temperature or 37 °C and then washed to … Se mer • Spectral traces for fluorescent dyes • Manual for Hoechst stains • An online guide to fluorescent probes and commercial labeling technologies Se mer Nettet30. jun. 2016 · Toxicity of Hoechst 33342: implication in side population analysis In the current study, we discovered that Hoechst 33342 (H342) could produce significant …

Hoechst 33258 C25H27Cl3N6O - PubChem

NettetOne of the most significant challenges in live imaging is to overcome photobleaching and phototoxicity. Excessive light exposure can cause cellular damage which may in turn … ebenefits proof of entitlement

Three-dimensional HepaRG spheroids as a liver model to study

NettetEvaluation of acute toxicity data after exposure via inhalation: There is a reliable disulphur dichloride study available conducted according to OECD guideline 403, well … NettetFor the Hoechst staining assay, cells were fixed with 4% paraformaldehyde for 15 min, then stained with Hoechst 33258 reagent at a concentration of 5 μg/mL for 10 min and washed with PBS. After drying, the nuclear morphology was observed with a confocal fluorescence microscope (Carl Zeiss LSM 710; Carl Zeiss Microscopy, Germany). NettetTwo supravital DNA dyes, Hoechst 33342 (H33342) and 4',6-diamidino-2-phenyl indole dihydrochloride (DAPI), were compared in terms of DNA histograms by flow cytometry … ebenefits preparation for decision phase